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FLIPR Potassium Assay KitsThe FLIPR® Potassium Assay Kit measures functional activity of ligand- and voltage-gated potassium channels. The homogeneous, no-wash assay protocol provides a large signal window and high Z’ values. The FLIPR Potassium Assay Kit exploits the permeability of thallium ions (Tl+) through both voltage- and ligand-gated potassium (K+) channels. In this assay, a novel, highly-sensitive Tl+ indicator dye is utilized which produces a bright fluorescent signal upon the binding to Tl+ conducted through potassium channels. The intensity of the Tl+ signal is proportional to the number of potassium channels in the open state; therefore it provides a functional indication of the potassium channel activities. In addition, one of our proprietary masking dyes is employed to further reduce background fluorescence for improved signal/noise ratio. - Functional measurement of potassium channel activity in a cell-based assay - Homogenous no-wash protocol reduces well-to-well variation and simplifies the workflow - Expanded signal window compared to non-homogenous assay - Rapid procedure with less hands‐on time
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